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(A) the expression levels of <t>CYP7A1</t> mRNA in the liver of normal control subjects (n=20) and diabetic patients (n=22) were quantified by TaqMan real-time PCR. (B) the representative Western blots exhibiting the protein expression of CYP7A1 and GAPDH in diabetic and control subjects. (C) quantification of CYP7A1 protein levels on Western blots. (D) the expression levels of BSEP mRNA in the liver of control subjects and diabetic patients were quantified by TaqMan real-time PCR. (E) the representative Western blots exhibiting the protein expression of BSEP and GAPDH in diabetic and control subjects. (F) quantification of BSEP protein levels on Western blots. For both real-time PCR and Western blotting, the expression levels of GAPDH were used to normalize the expression levels of CYP7A1 and BSEP mRNA and protein. The means and standard errors of the group values were indicated by the long and short lines, respectively. * p<0.05 with Student’s t-test.
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(A) the expression levels of CYP7A1 mRNA in the liver of normal control subjects (n=20) and diabetic patients (n=22) were quantified by TaqMan real-time PCR. (B) the representative Western blots exhibiting the protein expression of CYP7A1 and GAPDH in diabetic and control subjects. (C) quantification of CYP7A1 protein levels on Western blots. (D) the expression levels of BSEP mRNA in the liver of control subjects and diabetic patients were quantified by TaqMan real-time PCR. (E) the representative Western blots exhibiting the protein expression of BSEP and GAPDH in diabetic and control subjects. (F) quantification of BSEP protein levels on Western blots. For both real-time PCR and Western blotting, the expression levels of GAPDH were used to normalize the expression levels of CYP7A1 and BSEP mRNA and protein. The means and standard errors of the group values were indicated by the long and short lines, respectively. * p<0.05 with Student’s t-test.

Journal: Molecular and cellular endocrinology

Article Title: Dysregulation of Δ 4 -3-oxosteroid 5β-reductase in Diabetic Patients: Implications and Mechanisms

doi: 10.1016/j.mce.2017.10.005

Figure Lengend Snippet: (A) the expression levels of CYP7A1 mRNA in the liver of normal control subjects (n=20) and diabetic patients (n=22) were quantified by TaqMan real-time PCR. (B) the representative Western blots exhibiting the protein expression of CYP7A1 and GAPDH in diabetic and control subjects. (C) quantification of CYP7A1 protein levels on Western blots. (D) the expression levels of BSEP mRNA in the liver of control subjects and diabetic patients were quantified by TaqMan real-time PCR. (E) the representative Western blots exhibiting the protein expression of BSEP and GAPDH in diabetic and control subjects. (F) quantification of BSEP protein levels on Western blots. For both real-time PCR and Western blotting, the expression levels of GAPDH were used to normalize the expression levels of CYP7A1 and BSEP mRNA and protein. The means and standard errors of the group values were indicated by the long and short lines, respectively. * p<0.05 with Student’s t-test.

Article Snippet: Primary antibodies against AKR1D1 (sc-365932), CYP7A1 (sc-25536), BSEP (sc-25571), PPARα (sc-398394) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction, Western Blot

(A) in addition to CYP7A1, other key enzymes are involved in the classical and alternative bile acid synthesis pathways, including CYP8B1, CYP27A1 and CYP7B1. (B) the expression levels of CYP8B1, (B) CYP27A1 and (C) CYP7B1 mRNA in the liver of diabetic (n=20) and control subjects (n=18) were determined by TaqMan real-time PCR. The expression levels of GAPDH mRNA were used to normalize the expression levels of CYP8B1, CYP27A1 and CYP7B1. The means and standard deviations of the group values were indicated by the long and short lines, respectively. No statistical significances were detected by the Student’s t-test between normal and diabetic subjects.

Journal: Molecular and cellular endocrinology

Article Title: Dysregulation of Δ 4 -3-oxosteroid 5β-reductase in Diabetic Patients: Implications and Mechanisms

doi: 10.1016/j.mce.2017.10.005

Figure Lengend Snippet: (A) in addition to CYP7A1, other key enzymes are involved in the classical and alternative bile acid synthesis pathways, including CYP8B1, CYP27A1 and CYP7B1. (B) the expression levels of CYP8B1, (B) CYP27A1 and (C) CYP7B1 mRNA in the liver of diabetic (n=20) and control subjects (n=18) were determined by TaqMan real-time PCR. The expression levels of GAPDH mRNA were used to normalize the expression levels of CYP8B1, CYP27A1 and CYP7B1. The means and standard deviations of the group values were indicated by the long and short lines, respectively. No statistical significances were detected by the Student’s t-test between normal and diabetic subjects.

Article Snippet: Primary antibodies against AKR1D1 (sc-365932), CYP7A1 (sc-25536), BSEP (sc-25571), PPARα (sc-398394) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction